Journal: PLoS ONE

Article Title: Modified human glucagon-like peptide-1 (GLP-1) produced in E . coli has a long-acting therapeutic effect in type 2 diabetic mice

doi: 10.1371/journal.pone.0181939

Figure Lengend Snippet: MIN6 cells were cultured to a density of 6×10 4 cells/mL, then seeded into a 96-well flat plate (200 μL per well) and re-cultured for 24 hours. Control: cells treated with Dulbecco's phosphate buffered saline solution; Trypsin: cells treated with 0.25% trypsin; Trypsin + 6×mGLP-1: 10 μg/mL 6×mGLP-1 digested by 0.25% trypsin; GLP-1 standard: chemically synthesized native human GLP-1; Trypsin + GLP-1: 10 μg/mL GLP-1 digested by 0.25% trypsin. Cell density, measured as optical density at A490, was determined 48 h after the addition of the test compounds. The data represent the mean ± standard deviation (n = 6). The experiment was repeated three times and the results were statistically analyzed using one-way ANOVA method following Tukey post-hoc hypothesis test. The symbols a, b, and c refer to the significance level, and different symbol means statistical significance (at 5%, 1%, and 0.1% level) between treatments, or else, no significance.

Article Snippet: Based on the method described by Brandsma et al . [ ], with minor modifications, a mouse pancreatic tumor β cell line (MIN6) (iCell Bioscience Inc, Shanghai, China) was cultured and maintained in Roswell Park Memorial Institute (RPMI) 1640 medium containing 10% (v/v) fetal bovine serum, 100 U/mL penicillin, 0.1 mg/mL streptomycin and 50 μmol/L 2-mercaptoethanol at 37°C in an incubator with 5% CO 2 and 95% humidified air.

Techniques: Cell Culture, Control, Saline, Synthesized, Standard Deviation

Journal: Oncogenesis

Article Title: FAM83A is amplified and promotes cancer stem cell-like traits and chemoresistance in pancreatic cancer

doi: 10.1038/oncsis.2017.3

Figure Lengend Snippet: FAM83A is overexpressed in pancreatic cancer cell lines and primary human pancreatic cancer. ( a ) FAM83A mRNA expression in a published microarray data set (NCBI/GEO/GSE16515; contains 16 normal and 36 pancreatic tumor samples). ( b ) Kaplan–Meier analysis of overall (left) or disease-free (right) survival for patients with pancreatic cancer in the TCGA data set with low vs high FAM83A expression; * P <0.05. ( c , d ) Western blotting analysis of FAM83A expression in 2 primary normal human pancreatic duct epithelial cell (HPDEC) lines and 10 cultured pancreatic cancer cell lines ( c ) and 10 primary pancreatic cancer tissues (T) and the matched adjacent non-tumor tissues (ANT) ( d ); α-tubulin was used as protein loading control. ( e ) Immunohistochemical staining showing FAM83A protein expression was upregulated in human pancreatic cancer specimens compared with normal pancreatic tissues. ( f ) Kaplan–Meier overall (left) and disease-free (right) survival curves for patients with pancreatic cancer with low vs high FAM83A expression ( n =103; P <0.001, log-rank test).

Article Snippet: Primary cultures of normal human pancreatic duct epithelial cells were established from fresh specimens of the adjacent non-tumor pancreatic tissue and maintained in bronchial epithelial basal medium (Lonza Walkersville, Walkersville, MD, USA) containing 10% fetal bovine serum and supplemented with BEGM Single-Quots (Lonza Walkersville), according to previous reports., The human pancreatic cell lines AsPC-1, BxPC-3, Capan-1, Capan-2, CFPAC-1, Hs 766T, INS-1, MIA PaCa-2, MIN6 and PANC-1 were cultured as American Type Culture Collection (ATCC, Manassas, VA, USA) protocol described.

Techniques: Expressing, Microarray, Western Blot, Cell Culture, Control, Immunohistochemical staining, Staining