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Image Search Results
Journal: PLoS ONE
Article Title: Modified human glucagon-like peptide-1 (GLP-1) produced in E . coli has a long-acting therapeutic effect in type 2 diabetic mice
doi: 10.1371/journal.pone.0181939
Figure Lengend Snippet: MIN6 cells were cultured to a density of 6×10 4 cells/mL, then seeded into a 96-well flat plate (200 μL per well) and re-cultured for 24 hours. Control: cells treated with Dulbecco's phosphate buffered saline solution; Trypsin: cells treated with 0.25% trypsin; Trypsin + 6×mGLP-1: 10 μg/mL 6×mGLP-1 digested by 0.25% trypsin; GLP-1 standard: chemically synthesized native human GLP-1; Trypsin + GLP-1: 10 μg/mL GLP-1 digested by 0.25% trypsin. Cell density, measured as optical density at A490, was determined 48 h after the addition of the test compounds. The data represent the mean ± standard deviation (n = 6). The experiment was repeated three times and the results were statistically analyzed using one-way ANOVA method following Tukey post-hoc hypothesis test. The symbols a, b, and c refer to the significance level, and different symbol means statistical significance (at 5%, 1%, and 0.1% level) between treatments, or else, no significance.
Article Snippet: Based on the method described by Brandsma et al . [ ], with minor modifications, a
Techniques: Cell Culture, Control, Saline, Synthesized, Standard Deviation